Data/hora: 13/06/2024 23:59:33

Provedor de dados: 	86	
País: 	Brazil	
Título: 	Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration	
Autores: 	Houllou-Kido,Laureen Michelle; Kido,Ederson Akio; Falco,Maria Cristina; Silva Filho,Márcio de Castro; Figueira,Antônio Vargas de Oliveira; Nogueira,Neusa de Lima; Rossi,Mônica Lanzoni; Tulmann Neto,Augusto.	
Data: 	2005-11-01	
Ano: 	2005	
Palavras-chave: 	Musa; Cell suspension; Callus; Somatic differentiation; Biobalistic.	
Resumo: 	A plant regeneration method with cell suspension cultures of banana, and the effect of biobalistic on regeneration potential are described in this report. Somatic embryos of banana were obtained from indirect embryogenesis of male inflorescence of banana cultivar Maçã (AAB group). Part of the calluses formed (40%) showed embryogenic characteristics (nonfriable, compact and yellow color). The cell suspension, originated from embryogenic calluses, contained clusters of small tightly packed cells with dense cytoplasms, relatively large nuclei and very dense nucleoli. After four months of culture, somatic embryos started to regenerate. The maximum number of regenerated plants was observed between 45 and 60 days after embryo formation.In the first experiment, 401 plants were regenerated from approximately 10 mL of packed cells. In the second experiment, 399 plants were regenerated from a cell suspension six months older than that of the first experiment. Cell transformation using particle bombardment with three different plasmid constructions, containing the uid-A gene, resulted in a strong GUS expression five days after bombardment; however, plant regeneration from bombarded cells was much lower than nonbombarded ones.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001100005	
Editor: 	Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira	
Relação: 	10.1590/S0100-204X2005001100005	
Formato: 	text/html	
Fonte: 	Pesquisa Agropecuária Brasileira  v.40 n.11 2005	
Fonte: 	info:eu-repo/semantics/openAccess