Data/hora: 10/06/2024 00:42:02

Provedor de dados: | 86
País: | Brazil
Título: | Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
Autores: | Silva,Letícia Flores da; Guerra,Celito Crivellaro; Czermainski,Ana Beatriz Costa; Ferrari,Leonardo; Bergold,Ana Maria.
Data: | 2017-05-01
Ano: | 2017
Palavras-chave: | Vitis vinifera; High-pressure liquid chromatography; Polyphenol; Quantification.
Resumo: | Abstract: The objective of this work was to validate a fast method with low-solvent use, for the analysis of trans-resveratrol and quercetin in red wines. The wines were prepared, using a classical method, from the grape (Vitis vinifera) cultivars Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat, and Tempranillo. Samples were filtered and analyzed by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD), at 306 and 371 nm, for trans-resveratrol and quercetin, respectively. An octylsilane column was used, and the mobile phase was composed by a gradient of methanol, water, and formic acid. The method was validated according to the following figures of merit: specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The proposed HPLC-DAD method may be established for the analysis of trans-resveratrol and quercetin in red wines.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000500335

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/s0100-204x2017000500007
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.52 n.5 2017
Fonte: | info:eu-repo/semantics/openAccess