Data/hora: 12/06/2024 15:48:27

Provedor de dados: | 86
País: | Brazil
Título: | Short-term storage in vitro and large-scale propagation of grapevine genotypes
Autores: | Silva,Rafael de Carvalho; Luis,Zanderluce Gomes; Scherwinski-Pereira,Jonny Everson.
Data: | 2012-03-01
Ano: | 2012
Palavras-chave: | Vitis; Germplasm; Ex situ conservation; In vitro conservation; Micropropagation; Minimal growth.
Resumo: | The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 µmol L-1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20ºC, can be micropropagated in large scale.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000300005

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2012000300005
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.47 n.3 2012
Fonte: | info:eu-repo/semantics/openAccess