Data/hora: 10/06/2024 02:34:46

Provedor de dados: | 86
País: | Brazil
Título: | Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance
Autores: | Pinto,Fernanda de Oliveira; Maluf,Mirian Perez; Guerreiro-Filho,Oliveiro.
Data: | 2007-03-01
Ano: | 2007
Palavras-chave: | Leucoptera coffeella; Microsatellites; Molecular markers.
Resumo: | The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300011

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2007000300011
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.42 n.3 2007
Fonte: | info:eu-repo/semantics/openAccess