Data/hora: 09/06/2024 12:47:30

Provedor de dados: | Pesquisa Agropecuária Brasileira
País: | Brazil
Título: | Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato
Autores: | Amaral,Daniel Oliveira Jordão do; Lima,Marleide Magalhães de Andrade; Resende,Luciane Vilela; Silva,Márcia Vanusa da.
Data: | 2008-08-01
Ano: | 2008
Palavras-chave: | Lycopersicon esculentum; CDNA libraries; Complementary DNA; Gene expression; Salicylic acids; Suppression subtractive hybridization.
Resumo: | The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expressed. A total of 307 clones was identified in two subtractive libraries, which allowed the isolation of several defense-related genes that play roles in different mechanisms of plant resistance to phytopathogens. Genes with unknown roles were also isolated from the two libraries, which indicates the possibility of identifying new genes not yet reported in studies of stress/defense response. The SSH technique is effective for identification of resistance genes activated by salicylic acid and F. oxysporum f. sp. lycopersici infection. Not only the application of this technique enables a cost effective isolation of differentially expressed sequences, but also it allows the identification of novel sequences in tomato from a relative small number of sequences.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800010

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2008000800010
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.43 n.8 2008
Fonte: | info:eu-repo/semantics/openAccess