Data/hora: 04/06/2024 11:36:16

Provedor de dados: | 86
País: | Brazil
Título: | Agrobacterium-mediated genetic transformation of 'Hamlin' sweet orange
Autores: | Mendes,Beatriz Madalena Januzzi; Boscariol,Raquel Luciana; Mourão Filho,Francisco de Assis Alves; Almeida,Weliton Antonio Bastos de.
Data: | 2002-07-01
Ano: | 2002
Palavras-chave: | Citrus sinensis; Epicotyls; Seedlings; Transgenics; Breeding methods.
Resumo: | The development and optimization of efficient transformation protocols is essential in new citrus breeding programs, not only for rootstock, but also for scion improvement. Transgenic 'Hamlin' sweet orange (Citrus sinensis (L.) Osbeck) plants were obtained by Agrobacterium tumefaciens-mediated transformation of epicotyl segments collected from seedlings germinated in vitro. Factors influencing genetic transformation efficiency were evaluated including seedling incubation conditions, time of inoculation with Agrobacterium and co-culture conditions. Epicotyl segments were adequate explants for transformation, regenerating plants by direct organogenesis. Higher percentage of transformation was obtained with explants collected from seedlings germinated in darkness, transferred to 16 hours photoperiod for 2-3 weeks, and inoculated with Agrobacterium for 15-45 min. The best co-culture condition was the incubation of the explants in darkness, for three days in culture medium supplemented with 100 muM of acetosyringone. Genetic transformation was confirmed by performing beta-glucoronidase (GUS) assays and, subsequently, by PCR amplification for the nptII and GUS genes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000700009

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2002000700009
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.37 n.7 2002
Fonte: | info:eu-repo/semantics/openAccess