Data/hora: 10/06/2024 02:25:32

Provedor de dados: 	Pesquisa Agropecuária Brasileira	
País: 	Brazil	
Título: 	Tissue culture parameters in sweet orange cultivars	
Autores: 	Mendes,Amanda Ferreira da Silva; Cidade,Luciana Cardoso; Manzoli,Gabrielle Novais; Otoni,Wagner Campos; Soares Filho,Walter dos Santos; Costa,Marcio Gilberto Cardoso.	
Data: 	2008-08-01	
Ano: 	2008	
Palavras-chave: 	Citrus sinensis; Biotechnology; Gene transfer; Morphogenesis; Regeneration.	
Resumo: 	The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800020	
Editor: 	Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira	
Relação: 	10.1590/S0100-204X2008000800020	
Formato: 	text/html	
Fonte: 	Pesquisa Agropecuária Brasileira  v.43 n.8 2008	
Fonte: 	info:eu-repo/semantics/openAccess