Data/hora: 01/06/2024 16:28:04

Provedor de dados: 	Pesquisa Agropecuária Brasileira	
País: 	Brazil	
Título: 	Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter	
Autores: 	Attílio,Lísia Borges; Mourão Filho,Francisco de Assis Alves; Harakava,Ricardo; Silva,Tatiane Loureiro da; Miyata,Luzia Yuriko; Stipp,Liliane Cristina Libório; Mendes,Beatriz Madalena Januzzi.	
Data: 	2013-07-01	
Ano: 	2013	
Palavras-chave: 	Citrus sinensis; Antimicrobial peptide; Tissue‑specific promoter.	
Resumo: 	The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co‑cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real‑time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000700006	
Editor: 	Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira	
Relação: 	10.1590/S0100-204X2013000700006	
Formato: 	text/html	
Fonte: 	Pesquisa Agropecuária Brasileira  v.48 n.7 2013	
Fonte: 	info:eu-repo/semantics/openAccess