Data/hora: 16/06/2024 11:19:03

Provedor de dados: | 86
País: | Brazil
Título: | Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie
Autores: | Gonçalves,Aline Najara Domingos; Soares,Cleber Oliveira; Sanches,Simone Camargo; Reis,Fernando Alvarenga; Rosinha,Grácia Maria Soares.
Data: | 2016-05-01
Ano: | 2016
Palavras-chave: | Genotyping; PRNP gene; Single nucleotide polymorphisms; Transmissible spongiform encephalopathies.
Resumo: | Abstract: The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500684

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2016000500033
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.51 n.5 2016
Fonte: | info:eu-repo/semantics/openAccess