Data/hora: 10/06/2024 16:23:57

Provedor de dados: 	89	
País: 	Brazil	
Título: 	Increased iNOS gene expression in the granulosa layer of F1 follicle of over-fed and under-fed broiler breeder hens	
Autores: 	Sheikh Ahmadi,A; Zaghari,M; Shivazad,M; Hassanpour,H; Towhidi,A.	
Data: 	2010-12-01	
Ano: 	2010	
Palavras-chave: 	Broiler breeder; Follicle; Leptin-like hormone; Nitric oxide; Over/under-fed.	
Resumo: 	To clarify the effects of high (20 and 40% more than normal) and low (20% less than normal) daily feed allowance on egg and body parameters, ovarian morphology and plasma glucose, cholesterol, triacylglycerol, leptin-like hormone, nitrite/nitrate and gene expression of inducible nitric oxide synthase (iNOS) in the granulosa layer of F1 follicle, broiler breeder hens (30-week-old) were fed for 30 days. Egg and body parameters significantly changed between treatments (p&lt;0.05). Effect of different level of feed intake on ovarian morphology parameters was significant (p&lt;0.05), except for white follicles. After 30 days of experiment, plasma nitrite/nitrate (as a index of plasma nitric oxide) and leptin-like hormone increased in FI+20% and FI+40% groups as compared to controls (p&lt;0.05). Plasma level of leptin-like hormone also significantly (p&lt;0.05) increased in FI+40% group. The relative amount of iNOS mRNA expression in the granulosa layer of F1 follicles was significantly higher in FI-20% and FI+40% groups than in control group only after 4 weeks of experiment. The amount of these elevations in the FI-20% and FI+40% groups were 32.4% and 60.9% respectively. It was concluded that iNOS gene is normally expressed in follicular granulosa cells of F1 follicle of broiler breeder hens 2-4 hours before ovulation. However, over- and underfeeding of hens increased iNOS expression in F1 follicle, which may be one of the atresia-inducing factors in hierarchical follicles as shown by the significant (p&lt;0.05) increase of shrunken follicles in the ovary of over- and under-fed broiler breeder hens after 30 days of feeding.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000400004	
Editor: 	Fundação APINCO de Ciência e Tecnologia Avícolas	
Relação: 	10.1590/S1516-635X2010000400004	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Poultry Science  v.12 n.4 2010	
Fonte: 	info:eu-repo/semantics/openAccess