Data/hora: 13/06/2024 01:29:42

Provedor de dados: | 89
País: | Brazil
Título: | Evaluation of the Direct and Enrichment Iso 10272 Methods for the Detection of Campylobacter Spp. in Chicken Meat
Autores: | Benetti,TM; Abrahão,WM; Ferro,ID; Macedo,REF; Oliveira,TCRM.
Data: | 2016-03-01
Ano: | 2016
Palavras-chave: | Campylobacter spp.; Detection; ISO 10272; Refrigerated chicken carcass.
Resumo: | ABSTRACT In 2006, the International Organization for Standardization (ISO) published two standard protocols for the detection and enumeration of Campylobacter spp. in foods: the ISO 10272-2(direct method) and the ISO 10272-1 (enrichment method). The aim of this study was to compare the efficiency of these two methods in the detection of Campylobacter spp. and propose a modification in the enrichment method. Compared with the enrichment method, the direct method yielded a higher number of positive results for Campylobacter spp. and, consequently, presented higher sensitivity percentage. The isolation of Campylobacter spp. was more difficult when 10 mL of rinse was used in the enrichment method, which is currently recommended by the ISO protocol. Therefore, different rinse volumes were tested (2.5 and 5 mL). The most efficient recovery of Campylobacter spp. occurred when 2.5 mL of rinse were used in the enrichment method, most likely due to a lower number of microbial contaminants than that present in the 5 or 10 mL rinses. The proposed modification of the enrichment method will contribute to the food analysis by improving the detection of Campylobacter spp. in chicken carcass.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100023
Editor: | Fundação APINCO de Ciência e Tecnologia Avícolas
Relação: | 10.1590/1516-635x1801023-028
Formato: | text/html
Fonte: | Brazilian Journal of Poultry Science  v.18 n.1 2016
Fonte: | info:eu-repo/semantics/openAccess