Data/hora: 03/06/2024 02:27:56

Provedor de dados: | 89
País: | Brazil
Título: | Rotavirus detection and isolation from chickens with or without symptoms
Autores: | Villarreal,LYB; Uliana,G; Valenzuela,C; Chacón,JLV; Saidenberg,ABS; Sanches,AA; Brandão,PE; Jerez,JA; Ferreira,AJP.
Data: | 2006-09-01
Ano: | 2006
Palavras-chave: | Enteric viruses; Rotavirus; Isolation; MA104 cells; Chicken.
Resumo: | Rotaviruses have been identified as one of the main etiological agents of diarrhea and enteritis in mammals, including humans, and in avian species. Few studies have been published about enteric viruses in Brazilian poultry, including those related to rotavirus infection. Such studies demonstrate significant occurrence and the importance of enteric viruses in poultry presenting intestinal problems. Enteric viruses are the primary cause of injuries to the gut, allowing other agents, especially bacteria, to attach, to penetrate, and to replicate in the enteric tissue, leading to further damage. The aim of the present study was to detect rotavirus in the intestinal contents of layers and broilers by polyacrylamide gel electrophoresis (PAGE) and virus isolation in MA-104 cell culture. A total of 45.3% of all samples were positive to rotavirus; rotavirus frequencies were 48.7% in samples from flocks with diarrhea, 46.4% in flocks with delayed growth, and 30% in asymptomatic flocks. It was possible to isolate rotavirus in MA-104 cells from the nine rotavirus-positive randomly chosen samples. These results indicate that rotavirus may have an important role in pathogenesis of enteric disease.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000300009
Editor: | Fundação APINCO de Ciência e Tecnologia Avícolas
Relação: | 10.1590/S1516-635X2006000300009
Formato: | text/html
Fonte: | Brazilian Journal of Poultry Science  v.8 n.3 2006
Fonte: | info:eu-repo/semantics/openAccess