Data/hora: 02/06/2024 10:44:14

Provedor de dados: | 92
País: | Brazil
Título: | Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism
Autores: | Zaleski,Scheila R. M.; Lazzari,Sonia M. N.; Lazzarotto,Crisleide M.; Panzavolta,Tiziana; Iede,Edson T.; Marques,Francisco de A.
Data: | 2013-12-01
Ano: | 2013
Palavras-chave: | Banded pine weevil; Insecta; Molecular marker; Population genetics.
Resumo: | Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism. The objective of this study was to determine the genetic structure of populations of Pissodes castaneus from different areas and on different species of Pinus using the PCR-AFLP technique. Twenty samples were analyzed, representing 19 populations from Brazil and one from Florence, Italy, which is the region of origin of P. castaneus. The four combinations of primers generated a total of 367 fragments of DNA, and 100% of polymorphic loci, indicating high degree of molecular polymorphism. The dendrogram did not reveal trends for grouping the populations in relation to origin. The low genetic similarity (0.11 between the most distant groups) and genetic distances of 0.13 and 0.44 for 10 out of the 20 samples may indicate several founding events or multiple introductions of heterogeneous strains into Brazil. The allelic fixation index (Fst) was 0.3851, considered high, and the number of migrants (Nm) was 0.3991, indicating low gene flow among populations. The highest genetic distances were between the population from Irani, SC and Cambará do Sul, RS and Bituruna, PR, indicating an independent founding event or a particular allelic fixation in the former location. The high genetic diversity among populations points out that the populations are genetically heterogeneous with a diverse gene pool in the surveyed areas, what makes them to respond differently to control measures.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000400010
Editor: | Sociedade Brasileira De Entomologia
Relação: | 10.1590/S0085-56262013005000036
Formato: | text/html
Fonte: | Revista Brasileira de Entomologia  v.57 n.4 2013
Fonte: | info:eu-repo/semantics/openAccess