Data/hora: 09/06/2024 07:46:41

Provedor de dados: 	93	
País: 	Brazil	
Título: 	Isolation and partial purification of beta-galactosidases from cotyledons of two cowpea cultivars	
Autores: 	ENÉAS-FILHO,JOAQUIM; BARBOSA,GISLAINY KARLA DA COSTA; SUDÉRIO,FABRÍCIO BONFIM; PRISCO,JOSÉ TARQUÍNIO; GOMES-FILHO,ENÉAS.	
Data: 	2001-01-01	
Ano: 	2001	
Palavras-chave: 	Cotyledons; Isozymes; Vigna unguiculata; Vita 3 and Vita 5 cultivars; Salt tolerance.	
Resumo: 	Three isoforms of beta-galactosidases were isolated and partially purified from the cotyledons of quiescent seeds of Vita 3 and Vita 5 cowpea [Vigna unguiculata (L.) Walp.] cultivars differing in water and salt stress tolerance. The purification procedure consisted of ammonium sulfate fractionation, acid precipitation, ion exchange chromatography through DEAE-sephadex and affinity chromatography through Lactosyl-sepharose columns. The three isoforms isolated from the two cultivars showed the same chromatographic patterns, same optimum of temperature for enzyme activity assay (60ºC), identical thermal stability up to 50°C, and similar pH optima (3-4). However, they differed from each other in sensitivity towards metal ions and certain chemical agents presents in the assay medium. The results have shown that the observed differences in beta-galactosidases from the cotyledons of quiescent seeds were not sufficient to relate them to stress tolerance.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300001	
Editor: 	Sociedade Brasileira de Fisiologia Vegetal	
Relação: 	10.1590/S0103-31312001000300001	
Formato: 	text/html	
Fonte: 	Revista Brasileira de Fisiologia Vegetal  v.13 n.3 2001	
Fonte: 	info:eu-repo/semantics/openAccess