Data/hora: 13/06/2024 06:11:23

Provedor de dados: | 94
País: | Brazil
Título: | In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)
Autores: | Soares,Taliane Leila; Jesus,Onildo Nunes de; Santos-Serejo,Janay Almeida dos; Oliveira,Eder Jorge de.
Data: | 2013-12-01
Ano: | 2013
Palavras-chave: | Passion fruit; Pollen germination; Histochemical analysis; Wild passion fruit.
Resumo: | The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000400023
Editor: | Sociedade Brasileira de Fruticultura
Relação: | 10.1590/S0100-29452013000400023
Formato: | text/html
Fonte: | Revista Brasileira de Fruticultura  v.35 n.4 2013
Fonte: | info:eu-repo/semantics/openAccess