Data/hora: 09/06/2024 14:32:37

Provedor de dados: | 94
País: | Brazil
Título: | In vitro organogenesis of rangpur lime
Autores: | Soriano,Leonardo; Tavano,Eveline Carla da Rocha; Behling,Maurel; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi.
Data: | 2012-06-01
Ano: | 2012
Palavras-chave: | Adventitious shoot; Citrus limonia; Tissue culture.
Resumo: | Rangpur lime (Citrus limonia Osbeck) in vitro organogenesis was studied based on explant type and cytokinin culture media supplementation. Four explants types collected from epicotyl or hypocotyl regions of in vitro germinated seedlings were evaluated. The epicotyl-derived explants consisted of epicotyl segments and the hypocotyl-derived explants consisted of the entire hypocotyl segment, the hypocotyl segment attached to a cotyledon fragment, and the hypocotyl segment divided longitudinally. The explants were cultured on EME culture medium supplemented with benzylaminopurine (0, 0.5, 1.0, or 1.5 mg L-1). The evaluation was performed after 6 weeks. Best results considering both the explant responsiveness and number of shoots developed per explants were obtained when epicotyl segments-derived explants were evaluated. Considering the explant responsiveness of hypocotyl segments-derived explants no difference was detected between the entire hypocotyl segment and the hypocotyl segment attached to a cotyledon fragment. Moreover, the percentage of responsive explants decreased when hypocotyl segments divided longitudinally were tested. No difference was detected for the number of shoots developed per explant considering the three types of hypocotyl-derived explants. Culture media supplementation with BAP was not essential for Rangpur lime in vitro organogenesis. However, adventitious shoot development was stimulated in concentrations between 0.5 - 1.0 mg L-1.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452012000200006
Editor: | Sociedade Brasileira de Fruticultura
Relação: | 10.1590/S0100-29452012000200006
Formato: | text/html
Fonte: | Revista Brasileira de Fruticultura  v.34 n.2 2012
Fonte: | info:eu-repo/semantics/openAccess