Data/hora: 10/06/2024 02:30:03

Provedor de dados: | 94
País: | Brazil
Título: | Development of globular embryos from the hybridization between 'Pêra Rio' sweet orange and 'Poncã' mandarin
Autores: | Chagas,Edvan Alves; Pasqual,Moacir; Ramos,José Darlan; Cardoso,Pollyana; Cazetta,Jairo Osvaldo; Figueiredo,Milene Alves de.
Data: | 2003-12-01
Ano: | 2003
Palavras-chave: | Embryo culture; Tissue culture; Biotechnology; Citrus breeding.
Resumo: | This research was undertaken to study the influence of different concentrations of the MT medium, sucrose, vitamins, activated charcoal and gibberellic acid (GA3) on the culture of immature embryos from the crossing between 'Pêra Rio' sweet orange and 'PONCÃ' mandarin. The embryos were excised under aseptic conditions and inoculated in 15 mL of the MT medium according to the following experiments: 1) MT concentrations (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 2) vitamins concentrations of the MT (0%, 50%, 100%, 150% and 200%) supplemented with 0, 30, 60 and 90 g.L-1 of sucrose; 3) activated charcoal concentrations (0, 0.5, 1, 1.5 and 2 g.L-1) supplemented with GA3 (0, 0.01, 0.1; 1 and 10 mg.L-1). After the inoculation, the embryos were kept in a growth room for 90 days at 27 ± 1ºC, in a 16-hour photoperiod with 32 µmol.m-2.s-1 of irradiance. The best development of embryos at the globular stage was achieved using 50% and 100% of the MT medium plus 60 g.L-1 and 90 g.L-1 of sucrose, respectively, supplemented with 0.01 mg.L-1 of GA3. The addition of activated charcoal or vitamins in the MT medium has shown to be unnecessary to the development of globular embryos.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452003000300031
Editor: | Sociedade Brasileira de Fruticultura
Relação: | 10.1590/S0100-29452003000300031
Formato: | text/html
Fonte: | Revista Brasileira de Fruticultura  v.25 n.3 2003
Fonte: | info:eu-repo/semantics/openAccess