Data/hora: 10/06/2024 09:06:31

Provedor de dados: 	94	
País: 	Brazil	
Título: 	Self-incompatibility characterization in segregating populations of apple trees with DNA markers for S-alleles	
Autores: 	Brancher,Thyana Lays; Hawerroth,Maraisa Crestani; Hawerroth,Fernando José; Kvitschal,Marcus Vinícius; Denardi,Frederico; Guidolin,Altamir Frederico.	
Data: 	2021-01-01	
Ano: 	2021	
Palavras-chave: 	Malus × domestica Borkh; S genotype; S-RNase; Allele-specific PCR; Segregation.	
Resumo: 	Abstract  The objective of this study was to characterize the parents and respective populations of apple trees regarding S-alleles to confirm their genealogy and to evaluate the efficiency of the molecular markers used. Sixteen specific sets of primers were used for identification of apple S-alleles by PCR. Two segregating populations of the Epagri Apple Breeding Program resulting from crosses between ‘Fred Hough’ × ‘Monalisa’ and ‘M-11/00’ × ‘M-13/91’ were evaluated. The expected segregations are 1:1:1:1 for full compatibility and 1:1 for semi-compatibility, which can be confirmed by the X2 test. The ‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10) cross proved to be fully compatible; and two triploids were identified among the hybrids as well. The ‘M-11/00’ (S3S19) × ‘M-13/91’ (S3S5) cross was characterized as semi-compatible based on DNA markers, and the segregation of the S-alleles in the hybrids was 1:1, as expected. The segregation of the DNA markers occurred together with their respective S-alleles: S2, S3, S5, S10, and S19. Thus, characterization of the S-alleles not only allowed identification of compatibility between parents but also identified contaminations in segregating populations.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452021000100801	
Editor: 	Sociedade Brasileira de Fruticultura	
Relação: 	10.1590/0100-29452021167	
Formato: 	text/html	
Fonte: 	Revista Brasileira de Fruticultura  v.43 n.1 2021	
Fonte: 	info:eu-repo/semantics/openAccess