Data/hora: 09/06/2024 04:20:11

Provedor de dados: | Revista Brasileira de Sementes
País: | Brazil
Título: | Partial characterization of genes from the embryonic axis of Melanoxylon brauna Schott. (Leguminosae-Caesalpinioideae) seeds
Autores: | Silva,Mirian de Sousa; Brommonschenkel,Sérgio Hermínio; Faria,José Márcio Rocha; Borges,Eduardo Euclydes de Lima e.
Data: | 2012-01-01
Ano: | 2012
Palavras-chave: | Water; Stress; Drying; Sequencing.
Resumo: | The objective of this study was to partially characterize some genes involved in the desiccation tolerance of the embryonic axis of Melanoxylon brauna seeds subjected, or not, to oven fast-drying. Seeds were initially dried rapidly in an oven at 40 ºC, 50 ºC, 60 ºC, 70 ºC, and 80 °C, for 24, 48 and 72 h and then subjected to germination tests and moisture content determination. Degenerate primers were designed for 19 genes. The CDNA was used as a template for PCR amplifications using the degenerate primers, and the PCR products obtained were purified, cloned and sequenced. The seeds showed a gradual reduction in percent germination with increasing temperature and drying time. Nucleotide sequences of the cloned fragments related to genes CAT1, SPS1, Abi5, Transk and PM25 were obtained. The similarity analysis with the sequences deposited in databases revealed similarities with genes CAT1, SPS1, Transk and PM25 from other plant species. The nucleotide sequences obtained from the respective genes will be used for designing specific primers for gene expression analyses during seed germination in order to understand the causes for loss of physiological quality of Melanoxylon brauna seeds.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000100004
Editor: | Associação Brasileira de Tecnologia de Sementes
Relação: | 10.1590/S0101-31222012000100004
Formato: | text/html
Fonte: | Revista Brasileira de Sementes  v.34 n.1 2012
Fonte: | info:eu-repo/semantics/openAccess