Data/hora: 10/06/2024 08:20:47

Provedor de dados: | 96
País: | Brazil
Título: | In vitro free radicals scavenging activity and antioxidant capacity of solid-state fermented wheat bran and its potential modulation of antioxidative molecular targets in chicken PBMC
Autores: | Wang,Chaio-Chun; Chang,Chi-Huan; Chang,Shen-Chang; Fan,Geng-Jen; Lin,Min-Jung; Yu,Bi; Lee,Tzu-Tai.
Data: | 2016-08-01
Ano: | 2016
Palavras-chave: | Broiler; Feed additive; White rot fungi.
Resumo: | ABSTRACT The purpose of this study was to demonstrate the in vitro free radical scavenging activity and antioxidant capacity in solid-state fermented wheat bran and its potential modulation of antioxidative molecular targets in chicken peripheral blood mononuclear cells (PBMC). After solid-state fermentation of wheat bran by white rot fungi for 12 d, the scavenging action of the fermented wheat bran extracts was 1, 1-diphenyl-2-picrylhydrazyl (DPPH), and the free radicals increased significantly, approximately 1.5-fold. Trolox equivalent antioxidant capacity of 1 mg/mL fermented wheat bran extracts was increased from 100 to 150 mM trolox antioxidant capacity after 12 d of fermentation. Moreover, the extracts exhibited 50% of the chelating capacity observed for ferrous iron (Fe2+) after fermenting for 12 d. In vitro, and under the stimulus of fermented wheat bran, the antioxidant gene expression (GST, HO-1, Nrf2, and GCLC genes) of PBMC was more than double that of the PBS, ascorbic acid, and unfermented wheat bran. The expression of fermented wheat bran was the lowest for the NOX1 and ROMO1 genes. Solid-state wheat bran fermented by white rot fungi can increase the scavenging action of DPPH, the trolox equivalent antioxidant capacity, and the chelating capacity of ferrous iron; in addition, in vitro, it can regulate the expression of antioxidant molecular targets in chicken PBMC.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000800451
Editor: | Sociedade Brasileira de Zootecnia
Relação: | 10.1590/S1806-92902016000800005
Formato: | text/html
Fonte: | Revista Brasileira de Zootecnia  v.45 n.8 2016
Fonte: | info:eu-repo/semantics/openAccess