Data/hora: 04/06/2024 18:37:41

Provedor de dados: | 96
País: | Brazil
Título: | Effects of melanocortin 1 receptor (MC1R) gene polymorphisms on plumage color in mule ducks
Autores: | Tu,Yi-Chen; Wei,Liang-Yuan; Chang,Yi-Ying; Liu,Hsiu-Chou; Lee,Hsien-Hsiung; Yu,Yu-Hsiang; Chen,Ming-Cheng.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Muscovy drakes; Polymerase chain reaction-restriction fragment length polymorphism; Single nucleotide polymorphism; Tsaiya ducks.
Resumo: | ABSTRACT The objective of the present study was to investigate the effect of single nucleotide polymorphism (SNP) of the melanocortin 1 receptor (MC1R) gene on plumage coloration in mule ducks. PCR-high-resolution melting analysis (PCR-HRM) and DNA sequencing were used to identify the SNP variability of the MC1R gene in white common ducks. Three non-synonymous SNP (MC1R gene exon 1, c.52G&gt;A, c.376G&gt;A, and c.409G&gt;A) were identified in white Tsaiya ducks. Mating test (white Tsaiya ducks × white Muscovy drakes) in combination with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to investigate the effect of non-synonymous SNP of different maternal lines on plumage coloration in mule ducks. Genotyping results from 58 white Tsaiya ducks revealed the significant associations between genetic variations (c.52G&gt;A, c.376A&gt;G, and c.409G&gt;A) and plumage color in two maternal populations. After genotyping of 266 mule ducks, these three non-synonymous SNP identified in white Tsaiya ducks were significantly associated with plumage color of mule ducks. Therefore, the polymorphisms of MC1R gene at c.52G&gt;A, c.376A&gt;G, and c.409G&gt;A in white Tsaiya duck could be used in marker-assisted selection to improve the plumage color of mule ducks.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100305
Editor: | Sociedade Brasileira de Zootecnia
Relação: | 10.1590/rbz4820180180
Formato: | text/html
Fonte: | Revista Brasileira de Zootecnia  v.48 2019
Fonte: | info:eu-repo/semantics/openAccess