Data/hora: 10/06/2024 17:58:35

Provedor de dados: | Revista Brasileira de Zootecnia
País: | Brazil
Título: | Differential expression of genes in follicular cells of swines
Autores: | Miranda-Furtado,Cristiana Libardi; Silva,Priscila Vendramini; Guimarães,Marta Fonseca Martins; Serão,Nicola Vergara Lopes; Guimarães,José Domingos; Guimarães,Simone Eliza Facioni.
Data: | 2010-05-01
Ano: | 2010
Palavras-chave: | Candidate gene; Litter size; Ovulation rate; Pig production; Quantitative real-time PCR.
Resumo: | The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012
Editor: | Sociedade Brasileira de Zootecnia
Relação: | 10.1590/S1516-35982010000500012
Formato: | text/html
Fonte: | Revista Brasileira de Zootecnia  v.39 n.5 2010
Fonte: | info:eu-repo/semantics/openAccess