Data/hora: 03/06/2024 07:50:40

Provedor de dados: | Revista Brasileira de Zootecnia
País: | Brazil
Título: | Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
Autores: | Lew,Betina Joyce; Oliveira,Mauro Dal Secco de; Freitas Júnior,José Esler de; Carvalho,Marina Vieira de; Rêgo,Aníbal Coutinho do; Rennó,Francisco Palma.
Data: | 2013-07-01
Ano: | 2013
Palavras-chave: | Gene regulation; Mammary gland development; Microarray; Nutrition; Somatotropin.
Resumo: | The objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA microarray (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST). Significance levels of differential gene expression among treatments were assessed using a mixed model approach. Injection of bST altered the expression of 12 % of the genes on NBFGC slide related to tissue development, whereas 6% were altered by diet. Administration of bST increases the expression of genes positively related to cell proliferation and mammary parenchyma to a greater extent than a high energy diet.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000700008
Editor: | Sociedade Brasileira de Zootecnia
Relação: | 10.1590/S1516-35982013000700008
Formato: | text/html
Fonte: | Revista Brasileira de Zootecnia  v.42 n.7 2013
Fonte: | info:eu-repo/semantics/openAccess