Data/hora: 17/06/2024 14:38:34

Provedor de dados: | 98
País: | Cuba
Título: | Anxiogenic effects of Tabernaemontana solanifolia A. DC. (Apocynaceae) extracts in rats tested in the elevated plus-maze
Autores: | Mestriner Felipe Melo,Adriana Mary; Benitez Macorini,Luis Fernando; Müeller,André; Lima Cardoso,Cláudia Andréia; Gama,Stephanie; Gomes,Sueli Maria; Silveira,Dâmaris.
Data: | 2013-06-01
Ano: | 2013
Palavras-chave: | Elevated plus-maze; Anxiogenic; Tabernaemontana solanifolia; Apocynaceae.
Resumo: | Introduction: Tabernaemontana solanifolia A. DC. (Apocynaceae) grows in the Cerrado (savanna), Caatinga (shrublands), and Atlantic Forest of Brazil. Objective: to investigate the potential anxiolytic activity of the crude extracts and ß-amyrin acetate obtained from the leaves of Tabernaemontana solanifolia in the elevated plus-maze (EPM) test for anxiety in rats. Methods: the crude extracts (aqueous, ethanol, and hexane), as well as ß-amyrin acetate, obtained from Tabernaemontana solanifolia leaves were evaluated for possible anxiolytic effects in rats tested in the elevated plus-maze. Acute toxicity in rats was determined by OECD 423 guidelines. The leaves of T. solanifolia specimens collected in Brasília, Brazil, were air dried at 40 °C and macerated with hexane or ethanol. After filtration, the solvents were removed under reduced pressure, yielding the crude hexane and ethanolic extracts (5.96 and 18.62 % yield, respectively). The crude aqueous extract was obtained by infusion, followed by lyophilization (13.5 % yield). Thirty minutes before the elevated plus-maze experiments, the animals were treated with the crude ethanol extract (1 000 mg/kg of body weight [bw], p.o.), the crude hexane extract (1 000 mg/kg bw, p.o.), the crude extract (1 000 mg/kg bw, p.o.), or ß-amyrin acetate (21 mg/kg bw, p.o.). Results: the extracts cauded no mortality up to 2 000 mg/kg, so half of DL50 doses were selected for the present study. The crude extracts (hexane, ethanolic and aqueous) (1.0 g/kg) as well as the substance ß-amyrin acetate (21 mg/kg) were administered once, increasing the number of entries into and the time spent in the closed arms of the elevated plus-maze. Conclusion: the oral administration of the extracts and ß-amyrin acetate obtained from Tabernaemontana solanifolia had an anxiogenic effect in rats. Further studies are needed in order to identify and characterize the anxiogenic properties of Tabernaemontana solanifolia and to understand the anxiogenic mechanisms of the extracts.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962013000200005
Editor: | ECIMED
Formato: | text/html
Fonte: | Revista Cubana de Plantas Medicinales  v.18 n.2 2013