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Provedor de dados:  Trop. Plant Pathol.
País:  Brazil
Título:  AFLP markers differentiate isolates of Colletotrichum gossypii from C. gossypii var. cephalosporioides
Autores:  Silva-Mann,Renata
Vieira,Maria G. G. Carvalho
Machado,José C.
Bernardino Filho,José Roberto
Salgado,Kalinka C. C.
Stevens,Mikel R.
Data:  2005-04-01
Ano:  2005
Palavras-chave:  Molecular markers
Cotton diseases
Seed borne pathogens
Resumo:  Fungal diseases in cotton (Gossypium hirsutum), such as anthracnose caused by Colletotrichum gossypii and ramulose caused by C. gossypii var. cephalosporioides, are responsible for large yield losses. These pathogens are seed borne and morphologically similar although they induce different symptoms, which can lead to misdiagnosis using the blotter testing method. The present study was carried out to assess the viability of using Amplified Fragment Length Polymorphism (AFLP) markers to differentiate these pathogens. Five isolates, for each pathogen, were classified according to pathogenicity on cotton plants, and mycelial growth morphology. Conidial suspensions were sprayed on 30-day-old cotton plants and the symptoms assessed ten and 40 days after inoculation. For growth morphology 200 cottonseeds were inoculated with seven-day-old pure cultures, and the mycelial traits observed under a stereoscopic microscope seven days after inoculation. The DNA for AFLP analysis was obtained from seven-day-old fungal mycelia grown in liquid medium, using the Dneasy Qiagen protocol. Using the AFLP technique 318 polymorphic bands were selected to estimate similarities using Dice's Coefficient. The results clearly distinguished between ramulose and anthracnose isolates, which agreed with morphological and pathogenicity testing.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Fitopatologia
Relação:  10.1590/S0100-41582005000200011
Formato:  text/html
Fonte:  Fitopatologia Brasileira v.30 n.2 2005
Direitos:  info:eu-repo/semantics/openAccess

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