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Provedor de dados:  Rev. Bras. Zool.
País:  Brazil
Título:  Molecular cloning and expression patterns of the Vasa gene from Rana nigromaculata (Amphibia: Anura)
Autores:  Jia,Rui
Data:  2009-06-01
Ano:  2009
Palavras-chave:  DEAD-box
Phylogenetic analysis
Resumo:  The Vasa protein is a member of the DEAD (Asp-Glu-Alu-Asp) box family of ATP-dependent RNA helicases. The Vasa gene is specifically expressed in germ-line cells of many metazoans and is known to play a critical role in gametogenesis and reproductive regulation. In this paper, we isolate the full length cDNA sequence of the Vasa gene from the frog Rana nigromaculata Hallowell, 1861. The open reading frame (ORF) encoding 398 amino acid residues has nine conserved motifs. According to the similarities at the amino acid sequenceythe phylogenetic analysis of Vasa gene was consistent with the evolution relationships from chordates to mammals. Furthermore, the expression pattern analysis of RnVasa mRNA, using the technique of Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), showed a high level of transcripts in testis, ovary and kidney, whereas little to no signal was detected in other tissues, which suggests that it may play a role during gametogenesis.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Zoologia
Relação:  10.1590/S1984-46702009000200014
Formato:  text/html
Fonte:  Zoologia (Curitiba) v.26 n.2 2009
Direitos:  info:eu-repo/semantics/openAccess

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