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Provedor de dados:  Ciênc. Tecnol. Aliment.
País:  Brazil
Título:  Adhesion and production of degrading enzymes by bacteria isolated from biofilms in raw milk cooling tanks
Autores:  Flach,Juliana
Grzybowski,Valeska
Toniazzo,Geciane
Corção,Gertrudes
Data:  2014-09-01
Ano:  2014
Palavras-chave:  Psychrotrophic bacteria
Cleaning process
Lipase
Protease
Resumo:  Biofilms in milk cooling tanks compromise product quality even on farms. Due to the lack of studies of this topic, this study evaluated the microbiological conditions of raw milk cooling tanks on farms and characterized the microorganisms isolated from these tanks. Samples were wiped off with sterile swabs from seven milk cooling tanks in three different points in each tank. Mesophiles and psychrotrophic counts were performed in all samples. The isolation of Pseudomonas spp., Bacillus cereus and atypical colonies formed on selective media were also performed, totalizing 297 isolates. All isolates were tested for protease and lipase production and biofilm formation. Of the total isolates, 62.9% produced protease, 55.9% produced lipase, and 50.2% produced biofilm. The most widespread genus inside the milk cooling tank was Pseudomonas since it was not possible to associate this contamination with a single sampling point in the equipment. High counts of microorganisms were found in some cooling tanks, indicating poor cleaning of the equipment and providing strong evidences of microbial biofilm presence. Moreover, it is worth mentioning the milk potential contamination with both microbial cells and their degrading enzymes, which compromises milk quality.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000300020
Editor:  Sociedade Brasileira de Ciência e Tecnologia de Alimentos
Relação:  10.1590/1678-457x.6374
Formato:  text/html
Fonte:  Food Science and Technology v.34 n.3 2014
Direitos:  info:eu-repo/semantics/openAccess
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