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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers.
Autores:  MISSIO, R. F.
CAIXETA, E. T.
ZAMBOLIM, E. M.
PENA, G. F.
ZAMBOLIM, L.
DIAS, L. A. S.
SAKIYAMA, N. S.
Data:  2011-10-18
Ano:  2011
Palavras-chave:  Microsatellite marker
Coffea
Discriminant analysis
Genetic diversity
Resumo:  Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed with gSSR and EST-SSR markers. The study comprised 24 coffee accessions according to their genetic origin: arabica accessions (six traditional genotypes of C. arabica), resistant arabica (six leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C. arabica x C. canephora), triploids (three C. arabica x C. racemosa), and racemosa (one C. racemosa). Allele and polymorphism analysis, AMOVA, the Student t-test, Jaccard?s dissimilarity coefficient, cluster analysis, correlation of genetic distances, and discriminant analysis, were performed. EST-SSR markers gave 25 exclusive alleles per genetic group, while gSSR showed 47, which will be useful for differentiating accessions and for fingerprinting varieties. The gSSR markers detected a higher percentage of polymorphism among (35% higher on average) and within (42.9% higher on average) the genetic groups, compared to EST-SSR markers. The highest percentage of polymorphism within the genetic groups was found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%). It was possible to differentiate all genotypes including the arabica-related accessions. Nevertheless, combined use of gSSR and EST-SSR markers is recommended for coffee molecular characterization, because EST-SSRs can provide complementary information.

2011
Tipo:  Artigo em anais de congresso (ALICE)
Idioma:  Inglês
Identificador:  294

http://www.alice.cnptia.embrapa.br/handle/doc/903366

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/43671/1/Genetic-characterization-of-an-elite-coffee.pdf
Editor:  Genetics and Molecular Research, v. 10, n. 4, p 2366-2381, 2011.
Relação:  Embrapa Café - Artigo em anais de congresso (ALICE)
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