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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development.
Autores:  IVAMOTO, S. T.
SAKURAY, L. M.
FERREIRA, L. P.
KITZBERGER, C. S. G.
SCHOLZ, MARIA B. S.
POT, D.
LEROY, T.
VIEIRA, L. G. E.
DOMINGUES, D. S.
PEREIRA, L. F. P.
Data:  2017-04-13
Ano:  2016
Palavras-chave:  Cafestol
Kahweol
RT-qPCR
Expressão genética
Café
Composto químico
Lipidio
Biossíntese
Gene
Coffea Arábica.
Gene expression
High performance liquid chromatography.
Resumo:  Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30?240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways.
Tipo:  Artigo de periódico
Idioma:  Inglês
Identificador:  Plant Physiology and Biochemistry, v. 111, p. 340-347, Dec. 2016.

http://www.alice.cnptia.embrapa.br/alice/handle/doc/1068512
Direitos:  openAccess
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