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Provedor de dados:  Repositório Alice
País:  Brazil
Título:  Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil.
Autores:  TORRES JÚNIOR, C. V.
LEITE, J.
SANTOS, C. E. de R. e S.
FERNANDES JUNIOR, P. I.
ZILLI, J. E.
RUMJANEK, N. G.
XAVIER, G. R.
Data:  2014-04-01
Ano:  2014
Palavras-chave:  Arachis hypogaea
Fixação biológica de notrogênio
Rizóbio
Peanut
Amendoim
Resumo:  The use of peanut as a trap-plant for obtaining rhizobia may result in a high diversity of efficient isolates. This study aimed to evaluate the diversity and symbiotic efficiency of peanut rhizobia from the Southeastern region of Brazil. The bacteria were isolated from nodules of two genotypes in soils from the States of São Paulo and Rio de Janeiro, Brazil. The bacteria were authenticated and evaluated regarding to their symbiotic capacity. The rhizobial diversity was evaluated through their culture characteristics on yeast mannitol agar (YMA) culture media and by polymerase chain reaction Box- (PCR). Two selected isolates were characterized phenotypically regarding their capacity to solubilize phosphate, to grow on media with different concentrations of NaCI, pH's and incubation temperatures. For these the 16S rRNA gene sequencing and symbiotic efficiency using non-autoclaved soils were conducted. The majority of the obtained isolates showed rapid growth and acidified the culture medium. Analysis of the isolates through the Box-PCR revealed low similarity to the reference strain indicated for the culture and a large genetic variability of the obtained isolates. The isolates AM 01 and AM 07, which clustered with Bradyrhizobium and Rhizobium genus, respectively, showed a potential for evaluations and a means to recommend new rhizobia strains for peanut.

2014
Tipo:  Artigo em periódico indexado (ALICE)
Idioma:  Inglês
Identificador:  39441

http://www.alice.cnptia.embrapa.br/handle/doc/983750

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/118985/1/Diversity-and-symbiotic-performance-of-peanut.pdf

10.5897/AJMR 2013 . 5883
Editor:  African Journal of Microbiology Research v. 8 n. 6, p. 566-577, fev. 2014.
Relação:  Embrapa Agrobiologia - Artigo em periódico indexado (ALICE)
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