| Registro completo |
| Provedor de dados: |
Repositório Alice
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| País: |
Brazil
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| Título: |
Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene.
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| Autores: |
MOURA, R. R.
SOUZA-FABJAN, J. M. G.
FONSECA, J. F. da
MELO, C. H. S.
SANCHEZ, D. J. D.
VIEIRA, M. P.
ALMEIDA, T. M.
SEROVA, I. A.
SEROV, O. L.
PEREIRA, A. F.
TEIXEIRA, D. I. A.
MELO, L. M.
FREITAS, V. J. F.
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| Data: |
2014-05-02
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| Ano: |
2014
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| Palavras-chave: |
Transgenia
Goat.
Caprino
Cabra
Transferência de embrião
Hormônio animal
Progesterona
Superovulação
Embryo transfer
Progesterone
Superovulation
Transgenesis.
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| Resumo: |
Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene.
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| Tipo: |
Artigo de periódico
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| Idioma: |
Inglês
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| Identificador: |
Animal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/985516
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| Direitos: |
openAccess
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