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Provedor de dados:  Nature Precedings
País:  United Kingdom
Título:  Applying of novel subtracted method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes
Autores:  Ewa Siedlecka
Aneta Hromada-Judycka
Magdalena Pawełkowicz
Rafał Wóycicki
Monika Rakoczy-Trojanowska
Zbigniew Przybecki
Data:  2010-12-30
Ano:  2010
Palavras-chave:  Biotechnology
Developmental Biology
Genetics & Genomics
Plant Biology
Resumo:  We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate tags carrying the polymorphisms. 
To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for requested traits.
Tipo:  Manuscript
Fonte:  Nature Precedings
Direitos:  Creative Commons Attribution 3.0 License

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