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Provedor de dados:  Inra
País:  France
Título:  Transcript Profiling of Poplar Leaves upon Infection with Compatible and Incompatible Strains of the Foliar Rust Melampsora larici-populina
Autores:  Rinaldi, C.
Kohler, A.
Frey, P.
Duchaussoy, F
Ningre, F
Couloux, A.
Wincker, P
Le Thiec, D.
Fluch, S.
Martin, F.
Duplessis, S.
Data:  2007
Ano:  2007
Palavras-chave:  OBLIGATE BIOTROPHIC PATHOGEN
EXPRESSED SEQUENCE TAGS
FOR-GENE SPECIFICITY
HYPERSENSITIVE RESPONSE
HYBRID POPLAR
DISEASE RESISTANCE
LEAF RUST
FLAX RUST
AVIRULENCE GENES
GENOMIC SEQUENCE
Resumo:  To understand key processes governing defense mechanisms in poplar (Populus spp.) upon infection with the rust fungus Melampsora larici-populina, we used combined histological and molecular techniques to describe the infection of Populus trichocarpa X Populus deltoides 'Beaupre' leaves by compatible and incompatible fungal strains. Striking differences in host-tissue infection were observed after 48-h postinoculation (hpi) between compatible and incompatible interactions. No reactive oxygen species production could be detected at infection sites, while a strong accumulation of monolignols occurred in the incompatible interaction after 48 hpi, indicating a late plant response once the fungus already penetrated host cells to form haustorial infection structures. P. trichocarpa whole-genome expression oligoarrays and sequencing of cDNAs were used to determine changes in gene expression in both interactions at 48 hpi. Temporal expression profiling of infection-regulated transcripts was further compared by cDNA arrays and reverse transcription-quantitative polymerase chain reaction. Among 1,730 significantly differentially expressed transcripts in the incompatible interaction, 150 showed an increase in concentration >= 3-fold, whereas 62 were decreased by >= 3-fold. Regulated transcripts corresponded to known genes targeted by R genes in plant pathosystems, such as inositol-3-P synthase, glutathione S-transferases, and pathogenesis-related proteins. However, the transcript showing the highest rust-induced up-regulation encodes a putative secreted protein with no known function. In contrast, only a few transcripts showed an altered expression in the compatible interaction, suggesting a delay in defense response between incompatible and compatible interactions in poplar. This comprehensive analysis of early molecular responses of poplar to M. larici-populina infection identified key genes that likely contain the fungus proliferation in planta.
Tipo:  Journal Article
Idioma:  Inglês
Identificador:  http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20072887511d&uri=/notices/prodinra1/2011/04/

http://www.prodinra.inra.fr/prodinra/pinra/data/2011/04/PROD20072887511_20110408032800514.pdf
Formato:  application/pdf
Fonte:  Plant Physiology. 2007, 144 (1) : 347-366
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