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Provedor de dados:  Inra
País:  France
Título:  The glycolytic genes pfk and pyk from Lactobacillus casei are induced by sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system and repressed by CcpA
Autores:  Viana, R.
Pérez-Martinez, G.
Deutscher, J.
Monedero, V.
Data:  2005
Ano:  2005
Palavras-chave:  PTS
CATABOLITE REPRESSION
GLYCOLYSIS
PHOSPHOTRANSFERASE SYSTEM
CCPA
Resumo:  In Lactobacillus casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene (pfk) was cloned from L. casei and shown to be clustered with the gene encoding pyruvate kinase (pyk). pfk and pyk genes are cotranscribed and induced upon growth on sugars transported by the PTS. Contrarily to the model proposed for Lactococcus lactis, where the global catabolite regulator protein (CcpA) is involved in PTS-induced transcription of pfk and pyk, a ccpA mutation resulted in a slight increase in pfk–pyk expression in L. casei. This weak regulation was evidenced by CcpA binding to a region of the pfk–pyk promoter which contained two cre sequences significantly deviated from the consensus. The PTS induction of pfk–pyk seems to be counteracted by the CcpA-mediated repression. Our results suggest that the need to accommodate the levels of pfk–pyk mRNA to the availability of sugars is fulfilled in L. casei by a PTS/CcpA-mediated signal transduction different from L. lactis.
Tipo:  Journal Article
Idioma:  Inglês
Identificador:  http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2006bbba8d60&uri=/notices/prodinra1/2010/10/
Fonte:  Archives of Microbiology. 2005, 183 (6) : 385-393
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