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Provedor de dados:  OAK
País:  Japan
Título:  Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis
Autores:  Thekisoe, Oriel M. M.
Kuboki, Noritaka
Nambota, Andrew
Fujisaki, Kozo
Sugimoto, Chihiro
Igarashi, Ikuo
Yasuda, Jun
Inoue, Noboru
Data:  2007-06
Ano:  2007
Palavras-chave:  LAMP
Trypanosoma brucei brucei
T. b. rhodesiense
T. b. gambiense
T. congolense
T. cruzi
T. evansi
Resumo:  In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that lack sufficient resources needed for application of molecular diagnostic techniques.
Idioma:  Inglês
Editor:  Elsevier
Formato:  application/pdf

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