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Provedor de dados:  OMA
País:  Belgium
Título:  Uptake of <i>Vibrio anguillarum</i> vaccine by <i>Artemia salina</i> as a potential oral delivery system to fish fry
Autores:  Campbell, R.
Adams, A.
Tatner, M.F.
Chair, M.
Sorgeloos, P.
Data:  1993
Ano:  1993
Palavras-chave:  Artemia salina
Vibrio anguillarum
Resumo:  An ELISA to detect <i>Vibrio anguillarum</i> antigen in <i>Artemia salina</i> was developed. This assay was then used to determine the time course of antigen uptake by <i>Artemia</i> during incubations with <i>Vibrio</i> vaccine with a view to using <i>Artemia</i> as a vaccine carrier for oral vaccination of fish fry. The rate of uptake of vaccine by <i>Artemia</i> was shown to be concentration dependent. At a dose of 1.5x10<sup>7</sup> cfu ml<sup>-1</sup>, 1 mg wet weight of <i>Artemia</i> took up 1x10<sup>5</sup> cfu within 30min and reached maximum of 6x10<sup>6</sup> cfu after 60min. Thus each <i>Artemia</i> appears to have taken up approximately 1.2x10<sup>5</sup> cfu. Comparison of vaccine formulations indicated that uptake was consistently higher with formalised than with heat-treated vaccine.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2668
Fonte:  %3Ci%3EFish+Shellfish+Immunol.+3%286%29%3C%2Fi%3E%3A+451-459
Direitos:  info:eu-repo/semantics/restrictedAccess
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