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Provedor de dados:  OMA
País:  Belgium
Título:  Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (<i>Artemia</i> spp.) used for prophylactic chemotherapy of fish
Autores:  Nelis, H.
Léger, P.
Sorgeloos, P.
De Leenheer, A.P.
Data:  1991
Ano:  1991
Palavras-chave:  Aquaculture techniques
Fish culture
Infectious diseases
Resumo:  The brine shrimp <i>Artemia</i> , an excellent live food source in aquaculture, has been studied as a carrier to deliver selected chemotherapeutic agents to fish for prophylactic treatment of infectious diseases. To monitor the efficiency of incorporation of trimethoprim and sulfamethoxazole in <i><i>Artemia</i> franciscana</i>, a sensitive and specific analytical method was developed. It is based on homogenization of <i>Artemia</i> nauplii in methanol, extraction of lipids with hexane, solid-phase cleanup on C<sub>18</sub> cartridges, and reversed-phase liquid chromatography with detection at 210 nm. The method is sensitive (detection limit, on the order of 3 µg/g with a sample quantity of 30 mg [dry weight]) and reproducible (coefficients of variation, 2.2 and 1.8% for trimethoprim and sulfamethoxazole at levels of 79.6 and 257 µg/g of body weight, respectively). Preliminary quantitative data indicated excellent uptake and persistence of both therapeutic agents in <i>A. franciscana</i>, with levels of 115 µg/g for trimethoprim and 277 µg/g for sulfamethoxazole.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Formato:  application/pdf
Fonte:  %3Ci%3EAntimicrob.+Agents+Chemother.+35%2812%29%3C%2Fi%3E%3A+2486-2489
Direitos:  info:eu-repo/semantics/openAccess

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