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Provedor de dados:  Biological Sciences
País:  Brazil
Título:  Effects of glutathione supplementation and carbon source during somatic embryogenesis of Acca sellowiana (O.Berg) Burret (Myrtaceae)
Autores:  Pavei, Ana Flávia
Fraga, Hugo Pacheco de Freitas
Vieira, Leila do Nascimento
Guerra, Miguel Pedro
Data:  2018-04-01
Ano:  2018
Palavras-chave:  2.03.00.00-0 Botânica in vitro culture
Micropropagation
Somatic embryo conversion
Feijoa. 2.03.06.00-8 Botânica Aplicada
Resumo:   The present study intended to investigate the effects of different glutathione (GSH) levels (0, 0.1, 0.5 and 1 mM) on the somatic embryogenesis (SE) induction of Acca sellowiana. Besides, we evaluated the effect of different carbon sources (sucrose and maltose) on the somatic embryos conversion. GSH-supplemented treatments resulted in improved SE induction rates (~70%) as compared to the control GSH-free (~35%) after 50 days of culture. The total number of somatic embryos obtained did not differ between treatments, but significant differences were observed for the embryonic stages after 80 days of culture. After 80 days of culture, 0.5 and 1 mM GSH-supplemented treatments showed the largest amount of torpedo-staged somatic embryos. In contrast, treatments supplemented with 0 and 0.1 mM GSH showed equal amounts of somatic embryos at all embryonic stages. These results indicate that GSH accelerates the SE induction process and increases the synchrony of the somatic embryo formation of A. sellowiana. The use of maltose for the somatic embryos conversion, as compared to sucrose, did not influence the conversion rate of normal chlorophyllous somatic embryos, but increased the formation of normal achlorophyllous somatic plantlets. This finding can be attributed to the rapid hydrolysis of sucrose, contributing to an enhanced chlorophyll synthesis. 
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40257

10.4025/actascibiolsci.v40i1.40257
Editor:  Universidade Estadual De Maringá
Relação:  http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40257/pdf
Formato:  application/pdf
Fonte:  Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e40257

Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e40257

1807-863X

1679-9283
Direitos:  Direitos autorais 2018 Acta Scientiarum. Biological Sciences

http://creativecommons.org/licenses/by/4.0
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