Sabiia Seb
        Busca avançada

Botão Atualizar

Botão Atualizar

Registro completo
Provedor de dados:  Biological Sciences
País:  Brazil
Título:  Development and characterization of microsatellite markers for Tibouchina hatschbachii (Melastomataceae), an endemic and habitat-restricted shrub from Brazil
Autores:  Maia, Fabiano Rodrigo
Sujii, Patricia Sanae
Goldenberg, Renato
Pereira, Viviane Silva
Zucchi, Maria Imaculada
Data:  2016-12-08
Ano:  2016
Palavras-chave:  Genética Melastomataceae
Rock outcrops
Population genetics
Simple sequence repeat (SSR) Marcadores microssatélites
Resumo:  Tibouchina hatschbachii Wurdack (Melastomataceae) is an autogamous shrub restricted to granite (GO) and sandstone (SO) rock outcrops from subtropical Brazil. We designed primers for the amplification of microsatellite regions for T. hatschbachii, and characterized these primers to estimate genetic diversity parameters and contemporary genetic structure patterns. Eight loci were successfully amplified and were characterized using 70 individuals from three natural populations. Polymorphic information content ranged from 0.200 to 0.772 per locus. All loci were polymorphic, with allele numbers ranging from two to eight. The low degree of polymorphism may be explained by the fact that T. hatschbachii has disjunct populations and a recent genetic bottleneck, and also that it is self-pollinated. The observed and expected heterozygosities ranged from 0.115 to 1.000 and from 0.112 to 0.800, respectively. We observed private alleles in all loci. These are important features that enable us to identify population differentiation and help to us understand gene flow patterns for T. hatschbachii in subtropical Brazil. Eight microsatellite loci from other species of Tibouchina amplified positively in T. hatschbachii. 
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês

Editor:  Universidade Estadual De Maringá
Formato:  application/pdf
Fonte:  Acta Scientiarum. Biological Sciences; v. 38, n. 3 (2016); 327-332

Acta Scientiarum. Biological Sciences; v. 38, n. 3 (2016); 327-332


Direitos:  Direitos autorais 2016 Acta Scientiarum. Biological Sciences

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC:

Valid HTML 4.01 Transitional