| Registro completo |
| Provedor de dados: |
Anais da ABC (AABC)
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| País: |
Brazil
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| Título: |
Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China
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| Autores: |
Yu-bao,Cui
Zhou,Ying
Weihong,Shi
Guifang,Ma
Yang,Li
Yungang,Wang
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| Data: |
2010-12-01
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| Ano: |
2010
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| Palavras-chave: |
Dermatophagoides farinae
House-dust-mite allergy
Mite allergens
Der f 2
Allergen engineering
Immunotherapy
Bioinformatics
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| Resumo: |
To obtain the recombinant group 2 allergen product of Dermatophagoides farinae (Der f 2), the Der f 2 gene was synthesized by RT-PCR. The full-length cDNA comprised 441 nucleotides and was 99.3% identical to the reference sequence (GenBank AB195580). The cDNA was bound to vector pET28a to construct plasmid pET28a(+)-Der f 2, which was transformed into E. coli BL21 and induced by IPTG. SDS-PAGE showed a specific band of about 14kDa in the hole cell lysate. s estiated by chroatography, about 3.86 g of the recobinant product as obtained, which conjugated with serum IgE from asthmatic children. The protein had a signal peptide of 17 amino acids. Its secondary structure comprised an alpha helix (19.86%), an extended strand (30.82%), and a random coil (49.32%). The subcellular localization of this allergen was predicted to be at mitochondria. Furthermore, its function was shown to be associated with an MD-2-related lipid-recognition (ML) domain. The results of this study provide a solid foundation for large-scale production of the allergen for clinical diagnosis and treatent of allergic disorders.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400017
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| Editor: |
Academia Brasileira de Ciências
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| Relação: |
10.1590/S0001-37652010000400017
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| Formato: |
text/html
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| Fonte: |
Anais da Academia Brasileira de Ciências v.82 n.4 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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