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Provedor de dados:  Anais da ABC (AABC)
País:  Brazil
Título:  Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China
Autores:  Yu-bao,Cui
Data:  2010-12-01
Ano:  2010
Palavras-chave:  Dermatophagoides farinae
House-dust-mite allergy
Mite allergens
Der f 2
Allergen engineering
Resumo:  To obtain the recombinant group 2 allergen product of Dermatophagoides farinae (Der f 2), the Der f 2 gene was synthesized by RT-PCR. The full-length cDNA comprised 441 nucleotides and was 99.3% identical to the reference sequence (GenBank AB195580). The cDNA was bound to vector pET28a to construct plasmid pET28a(+)-Der f 2, which was transformed into E. coli BL21 and induced by IPTG. SDS-PAGE showed a specific band of about 14kDa in the hole cell lysate. s estiated by chroatography, about 3.86 g of the recobinant product as obtained, which conjugated with serum IgE from asthmatic children. The protein had a signal peptide of 17 amino acids. Its secondary structure comprised an alpha helix (19.86%), an extended strand (30.82%), and a random coil (49.32%). The subcellular localization of this allergen was predicted to be at mitochondria. Furthermore, its function was shown to be associated with an MD-2-related lipid-recognition (ML) domain. The results of this study provide a solid foundation for large-scale production of the allergen for clinical diagnosis and treatent of allergic disorders.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Academia Brasileira de Ciências
Relação:  10.1590/S0001-37652010000400017
Formato:  text/html
Fonte:  Anais da Academia Brasileira de Ciências v.82 n.4 2010
Direitos:  info:eu-repo/semantics/openAccess

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