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Provedor de dados:  Anais da ABC (AABC)
País:  Brazil
Título:  Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus
Autores:  Alves,Gutemberg G.
Marinho-Carvalho,Monica M.
Atella,Georgia C.
Silva-Neto,Mario A.C.
Data:  2007-03-01
Ano:  2007
Palavras-chave:  Phosphofructokinase
Resumo:  6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, and citrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activity for each tissue, with regulatory patterns that are consistent with their physiological roles.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Academia Brasileira de Ciências
Relação:  10.1590/S0001-37652007000100008
Formato:  text/html
Fonte:  Anais da Academia Brasileira de Ciências v.79 n.1 2007
Direitos:  info:eu-repo/semantics/openAccess

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