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Provedor de dados:  Anais da ABC (AABC)
País:  Brazil
Título:  Molecular identification of three species of Oncideres (Coleoptera: Cerambycidae) using RAPD markers
Autores:  CORDEIRO,GLÁUCIA
CUNHA,MARINA S. DA
SILVA,CAROLINA R. DA
JORGE,ISAAC R.
DERGAM,JORGE A.
FERREIRA,PAULO S.F.
Data:  2019-01-01
Ano:  2019
Palavras-chave:  Girdled branches
Insect identification
O. ocularis
O. saga
O. ulcerosa
Twig girdler
Resumo:  Abstract: Twig girdlers have a short adult life and a long larval stage (up to one year in some species). This fact, together with lack of morphological traits to identify the species from the larval stage, poses obstacles for the taxonomic identification and characterization of the many twig girdlers found in the wild. To solve this matter, Random Amplified Polymorphic DNA (RAPD) molecular markers have been applied to identify some insect species and to determine species boundaries. The aim of this study was to identify three species of Oncideres using the RAPD technique. Adults and larvae of O. saga, O. ocularis and O. ulcerosa were collected in Minas Gerais, Brazil. Two RAPD primers were used (OPA-05 and OPB-13), which produced different electrophoretic profiles that were used to construct a UPGMA phenogram. Three groups identified in the analyses accurately separated the three species, based on individuals in two different stages of development (adults and larvae): I: O. saga, II: O. ulcerosa, and III: O. ocularis. Molecular markers, such as RAPD, are valuable tools that help taxonomists in species identification.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500860
Editor:  Academia Brasileira de Ciências
Relação:  10.1590/0001-3765201920180209
Formato:  text/html
Fonte:  Anais da Academia Brasileira de Ciências v.91 n.3 2019
Direitos:  info:eu-repo/semantics/openAccess
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