| Registro completo |
| Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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| País: |
Brazil
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| Título: |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
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| Autores: |
Lima-Verde,I.B.
Matos,M.H.T.
Bruno,J.B.
Martins,F.S.
Santos,R.R.
Báo,S.N.
Luque,M.C.A.
Vieira,G.A.B.
Silveira,E.R.
Rodrigues,A.P.R.
Figueiredo,J.R.
Oliveira,M.A.L.
Lima,P.F.
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| Data: |
2009-02-01
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| Ano: |
2009
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| Palavras-chave: |
Caprine
Preantral follicle
Α-tocopherol
Ternatin
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| Resumo: |
The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.
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| Tipo: |
Info:eu-repo/semantics/report
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009
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| Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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| Relação: |
10.1590/S0102-09352009000100009
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| Formato: |
text/html
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| Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.61 n.1 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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