| Registro completo |
| Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
|
| País: |
Brazil
|
| Título: |
Addition of chlorogenic acid and caffeine during the processing of cooled boar semen
|
| Autores: |
Pereira,B.A.
Rocha,L.G.P.
Teles,M.C.
Silva,W.E.
Barbosa,J.A.
Rabelo,S.S.
Uchoa,A.S.
Rodrıguez-Gil,J. E.
Pereira,L.J.
Zangeronimo,M.G.
|
| Data: |
2019-04-01
|
| Ano: |
2019
|
| Palavras-chave: |
Antioxidant
Boar semen storage
Metabolic activator
Methylxanthine
Polyphenol
|
| Resumo: |
ABSTRACT A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200489
|
| Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
|
| Relação: |
10.1590/1678-4162-10415
|
| Formato: |
text/html
|
| Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.2 2019
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
|
