| Registro completo |
| Provedor de dados: |
47
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| País: |
Argentina
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| Título: |
Molecular cloning and characterization of a novel mannosebinding lectin cDNA from Zantedeschia aethiopica
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| Autores: |
Zhonghai,Chen
Yongzhen,Pang
Liu,Xiaojun
Wang,Xinglong
Deng,Zhongxiang
Sun,Xiaofen
Tang,Kexuan
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| Data: |
2005-08-01
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| Ano: |
2005
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| Palavras-chave: |
Mannose-binding lectin
RACE
Zantedeschia aethiopica
ZAA
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| Resumo: |
Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (zaa) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had many common characters of mannose-binding lectin superfamily and ZAAwas a mannose-binding lectin with three mannose-binding sites. Southern blot analysis of the genomic DNA revealed that zaa belonged to a multi-copy gene family.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200006
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| Editor: |
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
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| Formato: |
text/html
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| Fonte: |
Biocell v.29 n.2 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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