Registro completo |
Provedor de dados: |
Biol. Res.
|
País: |
Chile
|
Título: |
Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis
|
Autores: |
Wang,Hui
Liu,Zheng
Zhang,Wenxiu
Yuan,Ziao
Yuan,Hongyi
Liu,Xueting
Yang,Chunwen
Guan,Weijun
|
Data: |
2016-01-01
|
Ano: |
2016
|
Palavras-chave: |
Siberian tiger
Cadmium
Apoptosis
Fibroblast
Calcium
|
Resumo: |
BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 μΜ) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It also increased K+ efflux and altered the mRNA levels of Bax, Bcl-2, caspase-3, caspase-8, Fas, and p53. CONCLUSIONS: Our results suggest that Cd2+ triggers the apoptosis of Siberian tiger fibroblasts by disturbing intracellular homeostasis. These results will aid in our understanding of the effects of Cd2+ on Siberian tigers and in developing interventions to treat and prevent cadmium poisoning.
|
Tipo: |
Journal article
|
Idioma: |
Inglês
|
Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100042
|
Editor: |
Sociedad de Biología de Chile
|
Formato: |
text/html
|
Fonte: |
Biological Research v.49 2016
|
|