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Provedor de dados:  ArchiMer
País:  France
Título:  Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms
Autores:  Marti, R.
Mieszkin, Sophie
Solecki, O.
Pourcher, A. -m.
Hervio-heath, Dominique
Gourmelon, Michele
Data:  2011-11
Ano:  2011
Palavras-chave:  Lactobacillus amylovorus
Microbial source tracking
Pig-associated Bacteroidales markers
Quantitative real-time PCR
River water
Resumo:  Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20 degrees C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10 5, 8 1 and 17 2 days, respectively. Conclusions: Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters. Significance and Impact of the Study: The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters.
Tipo:  Text
Idioma:  Inglês

Editor:  Wiley-blackwell
Formato:  application/pdf
Fonte:  Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2011-11 , Vol. 111 , N. 5 , P. 1159-1175
Direitos:  2011 The Authors. Journal of Applied Microbiology 2011 The Society for Applied Microbiology

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