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Provedor de dados:  ArchiMer
País:  France
Título:  Purification and partial identification of novel antimicrobial protein from marine bacterium Pseudoalteromonas species strain x 4 53
Autores:  Longeon, Arlette
Peduzzi, Jean
Barthelemy, Michel
Corre, Sophie
Nicolas, Jean-louis
Guyot, Museum National D'Histoire Naturelle
Data:  2004-11
Ano:  2004
Palavras-chave:  Probiotic bacteria
Antimicrobial protein
Resumo:  A marine bacterium, X 153, was isolated from a pebble collected at St. Anne du Portzic (France). By 16S ribosomal DNA gene sequence analysis, X153 strain was identified as a Pseudoalteromonas sp. close to P. piscicida. The crude culture of X153 was highly active against human pathogenic strains involved in dermatologic diseases, and marine bacteria including various ichthyopathogenic Vibrio strains. The active substance occurred both in bacterial cells and in culture supernatant. An antimicrobial protein was purified to homogeneity by a 4-step procedure using size-exclusion and ion-exchange chromatography. The highly purified P-153 protein is anionic, and sodium dodecylsulfate polyacrylamide gel electrophoresis gives all apparent molecular mass of 87 kDa. The X153 bacterium protected bivalve larvae against mortality, following experimental challenges with ichthyopathogenic Vibrio. Pseudoalteromonas sp. X153 may be useful in aquaculture as a probiotic bacterium.
Tipo:  Text
Idioma:  Inglês

Editor:  Springer
Formato:  application/pdf
Fonte:  Marine Biotechnology (1436-2228) (Springer), 2004-11 , Vol. 6 , N. 6 , P. 633-641
Direitos:  Springer 2004

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