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Provedor de dados:  ArchiMer
País:  France
Título:  Morpho-molecular diversity and phylogeny of Bysmatrum (Dinophyceae) from the South China Sea and France
Autores:  Luo, Zhaohe
Lim, Zhen Fei
Mertens, Kenneth
Gurdebeke, Pieter
Bogus, Kara
Carbonell-moore, Maria Consuelo
Vrielinck, Henk
Leaw, Chui Pin
Lim, Po Teen
Chomerat, Nicolas
Li, Xintian
Gu, Haifeng
Data:  2018
Ano:  2018
Palavras-chave:  Benthic dinoflagellate
Bysmatrum granulosum
Bysmatrum gregarium
Bysmatrum subsalsum
Molecular phylogeny
Resumo:  The dinoflagellate genus Bysmatrum encompasses five epibenthic or tide-pool species and has been characterized by separated anterior intercalary plates. In the present study, we obtained six strains of Bysmatrum from the South China Sea and French Atlantic coast by isolating single cells/cysts from plankton and sediment samples. All strains were examined with light microscopy and scanning electron microscopy. Based on morphological observations, three strains were identified as Bysmatrum subsalsum, characterized by the elongated and rectangular first and a hexagonal second anterior intercalary plate. They differ from each other in the number of sulcal lists and the configuration of the first anterior intercalary plate. One strain was identified as Bysmatrum gregarium and the other two as Bysmatrum granulosum. The cyst-theca relationship of B. subsalsum from the French Atlantic was established by incubation of the cyst, and the geochemical composition of the cyst wall was measured through micro-Fourier transform infrared spectroscopy. Bysmatrum subsalsum from Malaysia shows a bright red stigma in the sulcal area under light microscopy, which was confirmed with transmission electron microscopy: it was identified as a type B eyespot. Small subunit ribosomal DNA (SSU rDNA), partial large subunit ribosomal DNA (LSU rDNA) and internal transcribed spacer (ITS) sequences were obtained from all six strains. The maximum likelihood and Bayesian inference analysis based on concatenated SSU, ITS and LSU sequences revealed that Bysmatrum is monophyletic and nested within Peridiniales. Our strains of B. subsalsum form a new ribotype in the molecular phylogeny (designated as ribotype B). The genetic distance based on ITS sequences among Bysmatrum species ranged from 0.34 to 0.47 and those genetic distances at the intraspecific level of B. subsalsum could reach 0.41, supporting the possibility of hidden crypticity within B. subsalsum.
Tipo:  Text
Idioma:  Inglês

Editor:  Taylor & Francis Ltd
Formato:  application/pdf
Fonte:  European Journal Of Phycology (0967-0262) (Taylor & Francis Ltd), 2018 , Vol. 53 , N. 3 , P. 318-335
Direitos:  2018 British Phycological Society


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