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Provedor de dados:  ArchiMer
País:  France
Título:  Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication
Autores:  Pauletto, Marianna
Segarra, Amelie
Montagnani, Caroline
Quillien, Virgile
Faury, Nicole
Le Grand, Jacqueline
Miner, Philippe
Petton, Bruno
Labreuche, Yannick
Fleury, Elodie
Fabioux, Caroline
Bargelloni, Luca
Renault, Tristan
Huvet, Arnaud
Data:  2017-10-18
Ano:  2017
Palavras-chave:  Anti-viral response
RNA interference
Inhibitor of NF-kappa B
Marine bivalve
Ostreid herpesvirus 1
Resumo:  Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters before being challenged by OsHV-1. Survival appeared close to 100% in both dsRNA-injected conditions associated with a low detection of viral DNA and a low expression of a panel of 39 OsHV-1 genes as compared with infected control. Long dsRNA molecules, both Cg-I kappa B2- and GFP-dsRNA, may have induced an anti-viral state controlling the OsHV-1 replication and precluding the understanding of the specific role of Cg-I kappa B2. Immune-related genes including Cg-I kappa B1, Cg-Rel1, Cg-IFI44, Cg-PKR and Cg-IAP appeared activated in the dsRNA-injected condition, potentially hampering viral replication and thus conferring a better resistance to OsHV-1 infection. We revealed that long dsRNA-mediated genetic interference triggered an anti-viral state in the oyster, emphasizing the need for new reverse genetics tools for assessing immune gene function and avoiding off-target effects in bivalves.
Tipo:  Text
Idioma:  Inglês

Editor:  Company Of Biologists Ltd
Relação:  info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED
Formato:  application/pdf
Fonte:  Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2017-10-18 , Vol. 220 , N. 20 , P. 3671-3685
Direitos:  2017. Published by The Company of Biologists Ltd


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